This article explores the valuable role of acupuncture as a supportive therapy during In vitro fertilization with embryo transfer (IVF-ET), demonstrating its ability to enhance success rates and increase the chances of pregnancy. From a modern biomedical perspective, acupuncture administered before and during the IVF cycle helps regulate the hypothalamic-pituitary-ovarian axis, promote hormonal balance, improve blood flow to the uterus and ovaries, and reduce psychological stress by inhibiting sympathetic activity. Frequently used acupoints in IVF support, typically located in the lower abdomen and middle to lower back, correspond anatomically with the innervation of the reproductive organs (T11–L2 and S2–S4 spinal nerves), suggesting a neurological basis for their synergistic effects. From a Traditional Chinese Medicine (TCM) view, treatment aligns with the Kidney-Tian Gui-Chong-Ren-Uterus axis, focusing on nourishing kidney essence and regulating menstruation. Optimal timing is key, suggesting pre-cycle treatment starting one to three months before IVF. Furthermore, research indicates that acupuncture performed specifically on the day of ET can significantly improve clinical pregnancy rates, especially in freeze-thaw cycles, and a higher pre-ET dosage is linked to increased live birth rates. By integrating both physiological and energetic aspects, acupuncture offers a holistic, evidence-informed approach to optimizing fertility.